ProLong Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging through use of enzymes from the plasma membrane of E. coli. These enzymes metabolize the elements that can cause photobleaching. When dyes are illuminated, they can degrade which reduces the sample’s fluorescent intensity and leads to the creation of free radical singlet oxygen which degrades neighboring dye molecules. ProLong Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours. ProLong Live reagent provides photobleach protection in live cell experiments for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker, LysoTracker, and CellTrackerdyes. ProLong Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.